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mouse antimouse col2a1 antibody  (Millipore)


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    Structured Review

    Millipore mouse antimouse col2a1 antibody
    Shown here are the intervertebral disc immunohistochemistry results of the novel bipedal standing mouse model. (A) The results showed that the degree of intervertebral disc degeneration in the experimental group was higher than that in the control group. (a-c) Specifically, the experimental group showed higher expression of collagen X in the cartilage endplate, (d-f) decreased expression of <t>Col2a1</t> in the annulus fibrosus, and (g-i) increased expression of MMP-13 and (j-l) OCN in the annulus fibrosus. (B) The statistical results of the ratio of collagen X-positive cells in the cartilage endplate are shown. (C) The statistical results of the ratio of MMP-13-positive cells in the annulus fibrosus are shown. (D) The statistical results of the ratio of OCN-positive cells in the annulus fibrosus are shown. *p < 0.05 compared with the control group; ▲p < 0.05 compared with the 6-week posttreatment group (scale bars = 50 μm [a-l]). CON = control group; POST6W = post-6-week treatment group; POST10W = post-10-week treatment group.
    Mouse Antimouse Col2a1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antimouse+col2a1+antibody/col2a1+antibody/pmc06554109-103-28-32
    Average 90 stars, based on 1 article reviews
    mouse antimouse col2a1 antibody - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration"

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration

    Journal: Clinical Orthopaedics and Related Research

    doi: 10.1097/CORR.0000000000000712

    Shown here are the intervertebral disc immunohistochemistry results of the novel bipedal standing mouse model. (A) The results showed that the degree of intervertebral disc degeneration in the experimental group was higher than that in the control group. (a-c) Specifically, the experimental group showed higher expression of collagen X in the cartilage endplate, (d-f) decreased expression of Col2a1 in the annulus fibrosus, and (g-i) increased expression of MMP-13 and (j-l) OCN in the annulus fibrosus. (B) The statistical results of the ratio of collagen X-positive cells in the cartilage endplate are shown. (C) The statistical results of the ratio of MMP-13-positive cells in the annulus fibrosus are shown. (D) The statistical results of the ratio of OCN-positive cells in the annulus fibrosus are shown. *p < 0.05 compared with the control group; ▲p < 0.05 compared with the 6-week posttreatment group (scale bars = 50 μm [a-l]). CON = control group; POST6W = post-6-week treatment group; POST10W = post-10-week treatment group.
    Figure Legend Snippet: Shown here are the intervertebral disc immunohistochemistry results of the novel bipedal standing mouse model. (A) The results showed that the degree of intervertebral disc degeneration in the experimental group was higher than that in the control group. (a-c) Specifically, the experimental group showed higher expression of collagen X in the cartilage endplate, (d-f) decreased expression of Col2a1 in the annulus fibrosus, and (g-i) increased expression of MMP-13 and (j-l) OCN in the annulus fibrosus. (B) The statistical results of the ratio of collagen X-positive cells in the cartilage endplate are shown. (C) The statistical results of the ratio of MMP-13-positive cells in the annulus fibrosus are shown. (D) The statistical results of the ratio of OCN-positive cells in the annulus fibrosus are shown. *p < 0.05 compared with the control group; ▲p < 0.05 compared with the 6-week posttreatment group (scale bars = 50 μm [a-l]). CON = control group; POST6W = post-6-week treatment group; POST10W = post-10-week treatment group.

    Techniques Used: Immunohistochemistry, Expressing

    Related Articles

    Incubation:

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16 IKN4A , aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany). ..

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16IKN4A, aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the Copyright © 2019 by the Association of Bone and Joint Surgeons. .. Unauthorized reproduction of this article is prohibited. corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany).

    Staining:

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16 IKN4A , aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany). ..

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16IKN4A, aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the Copyright © 2019 by the Association of Bone and Joint Surgeons. .. Unauthorized reproduction of this article is prohibited. corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany).

    Expressing:

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16 IKN4A , aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany). ..

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16IKN4A, aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the Copyright © 2019 by the Association of Bone and Joint Surgeons. .. Unauthorized reproduction of this article is prohibited. corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany).

    Light Microscopy:

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration
    Article Snippet: .. The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16 IKN4A , aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany). ..



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    Millipore mouse antimouse col2a1 antibody
    Shown here are the intervertebral disc immunohistochemistry results of the novel bipedal standing mouse model. (A) The results showed that the degree of intervertebral disc degeneration in the experimental group was higher than that in the control group. (a-c) Specifically, the experimental group showed higher expression of collagen X in the cartilage endplate, (d-f) decreased expression of <t>Col2a1</t> in the annulus fibrosus, and (g-i) increased expression of MMP-13 and (j-l) OCN in the annulus fibrosus. (B) The statistical results of the ratio of collagen X-positive cells in the cartilage endplate are shown. (C) The statistical results of the ratio of MMP-13-positive cells in the annulus fibrosus are shown. (D) The statistical results of the ratio of OCN-positive cells in the annulus fibrosus are shown. *p < 0.05 compared with the control group; ▲p < 0.05 compared with the 6-week posttreatment group (scale bars = 50 μm [a-l]). CON = control group; POST6W = post-6-week treatment group; POST10W = post-10-week treatment group.
    Mouse Antimouse Col2a1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antimouse+col2a1+antibody/col2a1+antibody/pmc06554109-103-28-32
    Average 90 stars, based on 1 article reviews
    mouse antimouse col2a1 antibody - by Bioz Stars, 2026-09
    90/100 stars
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    Shown here are the intervertebral disc immunohistochemistry results of the novel bipedal standing mouse model. (A) The results showed that the degree of intervertebral disc degeneration in the experimental group was higher than that in the control group. (a-c) Specifically, the experimental group showed higher expression of collagen X in the cartilage endplate, (d-f) decreased expression of Col2a1 in the annulus fibrosus, and (g-i) increased expression of MMP-13 and (j-l) OCN in the annulus fibrosus. (B) The statistical results of the ratio of collagen X-positive cells in the cartilage endplate are shown. (C) The statistical results of the ratio of MMP-13-positive cells in the annulus fibrosus are shown. (D) The statistical results of the ratio of OCN-positive cells in the annulus fibrosus are shown. *p < 0.05 compared with the control group; ▲p < 0.05 compared with the 6-week posttreatment group (scale bars = 50 μm [a-l]). CON = control group; POST6W = post-6-week treatment group; POST10W = post-10-week treatment group.

    Journal: Clinical Orthopaedics and Related Research

    Article Title: Development and Characterization of a Novel Bipedal Standing Mouse Model of Intervertebral Disc and Facet Joint Degeneration

    doi: 10.1097/CORR.0000000000000712

    Figure Lengend Snippet: Shown here are the intervertebral disc immunohistochemistry results of the novel bipedal standing mouse model. (A) The results showed that the degree of intervertebral disc degeneration in the experimental group was higher than that in the control group. (a-c) Specifically, the experimental group showed higher expression of collagen X in the cartilage endplate, (d-f) decreased expression of Col2a1 in the annulus fibrosus, and (g-i) increased expression of MMP-13 and (j-l) OCN in the annulus fibrosus. (B) The statistical results of the ratio of collagen X-positive cells in the cartilage endplate are shown. (C) The statistical results of the ratio of MMP-13-positive cells in the annulus fibrosus are shown. (D) The statistical results of the ratio of OCN-positive cells in the annulus fibrosus are shown. *p < 0.05 compared with the control group; ▲p < 0.05 compared with the 6-week posttreatment group (scale bars = 50 μm [a-l]). CON = control group; POST6W = post-6-week treatment group; POST10W = post-10-week treatment group.

    Article Snippet: The sections were then incubated with rabbit antimouse osteocalcin (OCN; ABclonal, Wuhan, China), matrix metalloprotease-13 (MMP-13), collagen X, P16 IKN4A , aggrecan, and vimentin antibodies(Abcam, Cambridge, USA), and mouse antimouse Col2a1 antibody (Millipore, Burlington, MA, USA) at 4° C overnight followed by incubation in secondary antibody (ABclonal) for 1 hour at room temperature, DAB chromogenesis, H&E staining, and mounting to observe the expression of the corresponding proteins using light microscopy (Axio Scope.A1, Zeiss, Jena, Germany).

    Techniques: Immunohistochemistry, Expressing